| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 591 | Bacillus smithii | ACGCCTTGGAAAACCGGT | ACGCAAAGGCAGCTTCCA | 59.81 | 60.20 | 243 | 84 |
100.00%
|
100.00%
|
| Primer 592 | Bacillus smithii | CATTGTGGGGTGTGGTGGA | ATGCTGTCCCTACAACGGC | 59.85 | 60.08 | 192 | 84 |
100.00%
|
100.00%
|
| Primer 593 | Bacillus smithii | CAGACCGTGGGGATTTGGT | TTCGGGTGGTGCTTGATCC | 59.62 | 60.00 | 275 | 84 |
100.00%
|
100.00%
|
| Primer 594 | Bacillus smithii | TCTCCGTGACGCCATTGT | CAGTTCAGTCGCTTGGGGT | 58.94 | 59.93 | 241 | 84 |
100.00%
|
100.00%
|
| Primer 595 | Bacillus smithii | TGTTCTCCGTGACGCCAT | CAGTTCAGTCGCTTGGGGT | 58.94 | 59.93 | 244 | 84 |
100.00%
|
100.00%
|
| Primer 596 | Bacillus smithii | ATGTTCTCCGTGACGCCA | CAGTTCAGTCGCTTGGGGT | 58.94 | 59.93 | 245 | 84 |
100.00%
|
100.00%
|
| Primer 597 | Bacillus smithii | AGGGATGGCAATGGCGAAA | ACGTTGCTTTGCCGAGGT | 60.00 | 60.20 | 221 | 84 |
100.00%
|
100.00%
|
| Primer 598 | Bacillus smithii | TGGAGTGGCATGCAGTCTG | ACGTTGCTTTGCCGAGGT | 60.00 | 60.20 | 296 | 84 |
100.00%
|
100.00%
|
| Primer 599 | Bacillus smithii | CATGAGCCGTTCAGGGGAT | GCATTCGGCTTTCGCGAA | 59.47 | 59.52 | 260 | 84 |
100.00%
|
100.00%
|
| Primer 600 | Bacillus smithii | TGAGCCGTTCAGGGGATTC | GCATTCGGCTTTCGCGAA | 59.40 | 59.52 | 258 | 84 |
100.00%
|
100.00%
|